Journal: Cells
Article Title: Protective Effects of Keratinocyte-Derived GCSF and CCL20 on UVB-Induced Melanocyte Damage
doi: 10.3390/cells13191661
Figure Lengend Snippet: Epidermal thickness and the expression of GCSF, CCL20, and tyrosinase protein in UVB-treated mouse skin. Epidermal thickness ( A ), immunofluorescence of GCSF ( A ), CCL20 ( C ), tyrosinase ( A , C ), and pan-cytokeratin, a marker that identifies keratinocytes in all layers of the epidermis ( A , C ), were assessed 12 h following the final UVB exposure. Red arrows indicate the epidermal layer, while white arrows mark the dermal-epidermal junction, delineating the boundary between the epidermis and dermis. The summary graph shows the statistical analysis of epidermal thickness ( B ), and the relative protein levels of GCSF ( D ), and CCL20 ( E ), to pan-cytokeratin and tyrosinase ( F ). The data were quantified using ImageJ and GraphPad Prism software (v9) and are expressed as mean ± SD, with 1 N (dot) representing one area from four areas in a mouse. A mouse provides three N. The statistical significance of differences was evaluated by one-way ANOVA followed by Dunnett’s test. *** p < 0.001 versus non-irradiated group.
Article Snippet: Primary human epidermal melanocytes (MC) and primary human epidermal keratinocytes (KC) were obtained from Invitrogen (Waltham, MA, USA).
Techniques: Expressing, Immunofluorescence, Marker, Software, Irradiation